PLA2G4A Antibody

• Catalog Number: orb20577
• Category: Antibodies
• Description: Goat polyclonal antibody to PLA2G4A
• Target: PLA2G4A
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Human
• Predicted Reactivity: Mouse, Rat
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Protein Sequence: QEKTFRQQRKEHIR
• RRID: AB_10753888
• MW: 85.2
• Tested applications: ELISA, FC, IF, IHC, WB
• Dilution range: ELISA: 1:32000, WB: 0.3-1 μg/ml, IHC-P: 3.75 μg/ml
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.WB: Approx 90kDa band observed in lysates of cell lines HeLa and NIH3T3 (calculated MW of 85.2kDa according to NP_077734.1). Recommended concentration: 1-3 μg/ml.
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: anti calcium-dependent phospholipid-binding protei
• Research Area: Cardiovascular Disease, Epigenetics
• Note: For research use only
• Entrez: 5321

orb20577 (1ug/ml) staining of HeLa lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Immunofluorescence analysis of A549 cells using PLA2G4A antibody

Flow cytometric analysis of A549 cells using PLA2G4A antibody

Immunohistochemical staining of Human Uterus using PLA2G4A antibody.