PLA2G4A Antibody

SKU: orb20577

Description

Goat polyclonal antibody to PLA2G4A

Images & Validation

• Tested Applications: ELISA, FC, IF, IHC, WB
• Dilution range: ELISA: 1:32000, WB: 0.3-1 μg/ml, IHC-P: 3.75 μg/ml
• Reactivity: Human
• Predicted Reactivity: Mouse, Rat
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.WB: Approx 90kDa band observed in lysates of cell lines HeLa and NIH3T3 (calculated MW of 85.2kDa according to NP_077734.1). Recommended concentration: 1-3 μg/ml.

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: PLA2G4A
• Molecular Weight: 85.2
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Disclaimer: For research use only

Alternative Names

anti calcium-dependent phospholipid-binding protein antibody, anti cPLA2-alpha antibody, anti cytosolic phospholipase A2, group IVA antibody, anti lysophospholipase antibody, anti MGC126350 antibody, anti phosphatidylcholine 2-acylhydrolase antibody, anti phospholipase A2, group IVA (cytosolic, calcium-dependent) antibody, anti PLA2G4 antibody, anti PLA2G4A antibody

orb20577 (1ug/ml) staining of HeLa lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Immunofluorescence analysis of A549 cells using PLA2G4A antibody

Flow cytometric analysis of A549 cells using PLA2G4A antibody

Immunohistochemical staining of Human Uterus using PLA2G4A antibody.

Quick Database Links

Gene Symbol

PLA2G4A

NCBI Gene ID

5321