PLA2G1B Antibody

• Catalog Number: orb19655
• Category: Antibodies
• Description: Goat polyclonal antibody to PLA2G1B
• Target: PLA2G1B
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Human
• Predicted Reactivity: Canine
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Protein Sequence: NKAHKNLDTKKYCQS
• RRID: AB_10753578
• MW: 16.4
• Tested applications: ELISA, WB
• Dilution range: ELISA: 1:64000, WB: 0.01 μg/ml
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:64000.WB: Approx. 17-18kDa band observed in Human Pancreas lysates (calculated MW of 16.4kDa according to NP_000919.1). In transfected HEK293 transiently expressing PLA2G1B a band of approx. 18kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0.01 μg/ml.
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: anti PLA2G1B antibody, anti phospholipase A2, grou
• Research Area: Cardiovascular Disease, Epigenetics
• Note: For research use only
• Entrez: 5319
• Expiration Date: 12 months from date of receipt.

orb19655 (0.01ug/ml) staining of Human Pancreas lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

HEK293 overexpressing PLA2G1B (RC216089) and probed with orb19655 (mock transfection in first lane), tested by Origene.