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Catalog Number | orb19655 |
---|---|
Category | Antibodies |
Description | Goat polyclonal antibody to PLA2G1B |
Species/Host | Goat |
Clonality | Polyclonal |
Tested applications | ELISA, WB |
Reactivity | Canine, Human |
Dilution range | ELISA: 1:64000, WB: 0.01 μg/ml |
Conjugation | Unconjugated |
MW | 16.4 |
Target | PLA2G1B |
Entrez | 5319 |
Protein Sequence | NKAHKNLDTKKYCQS |
RRID | AB_10753578 |
Storage | Aliquot and store at -20°C. Minimize freezing and thawing. |
Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
Alternative names | anti PLA2G1B antibody, anti phospholipase A2, grou Read more... |
Note | For research use only |
Application notes | ELISA: Peptide ELISA: antibody detection limit dilution 1:64000.WB: Approx. 17-18kDa band observed in Human Pancreas lysates (calculated MW of 16.4kDa according to NP_000919.1). In transfected HEK293 transiently expressing PLA2G1B a band of approx. 18kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0.01 μg/ml. |
Expiration Date | 12 months from date of receipt. |
orb19655 (0.01ug/ml) staining of Human Pancreas lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
HEK293 overexpressing PLA2G1B (RC216089) and probed with orb19655 (mock transfection in first lane), tested by Origene.