PLA2G1B Antibody

SKU: orb19655

Description

Goat polyclonal antibody to PLA2G1B

Research Area

Metabolism Research

Images & Validation

• Tested Applications: ELISA, WB
• Dilution Range: ELISA: 1:64000, WB: 0.01 μg/ml
• Reactivity: Human
• Predicted Reactivity: Canine
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:64000.WB: Approx. 17-18kDa band observed in Human Pancreas lysates (calculated MW of 16.4kDa according to NP_000919.1). In transfected HEK293 transiently expressing PLA2G1B a band of approx. 18kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0.01 μg/ml.

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: PLA2G1B
• Protein Sequence: NKAHKNLDTKKYCQS
• Molecular Weight: 16.4
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti PLA2G1B antibody, anti phospholipase A2, group IB (pancreas) antibody, anti MGC119834 antibody, anti MGC119835 antibody, anti PLA2 antibody, anti PLA2A antibody, anti PPLA2 antibody, anti phospholipase A2, group IB antibody

orb19655 (0.01ug/ml) staining of Human Pancreas lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

HEK293 overexpressing PLA2G1B (RC216089) and probed with orb19655 (mock transfection in first lane), tested by Origene.

Quick Database Links

Gene Symbol

PLA2G1B

NCBI Gene ID

5319