PIWI/HIWI Antibody

• Catalog Number: orb19551
• Category: Antibodies
• Description: Goat polyclonal to PIWI
• Target: PIWI / HIWI
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Human
• Predicted Reactivity: Bovine, Canine, Mouse, Porcine, Rat
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Protein Sequence: SNRKDKYDAIKKY
• RRID: AB_10749922
• MW: 98.6; 94.8
• Tested applications: ELISA, FC, IF, IHC
• Dilution range: ELISA: 1:64000, WB: 0.1 μg/ml, IHC-P: 4-6 μg/ml
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:64000.WB: Preliminary experiments showed a 100kDa band but also bands at approx 35kDa and 26-28kDa in Human Ovary, Placenta, Prostate and Testis lysates after 0.1 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the additional bands we observe given the calculated size of 98.6kDa according to NP_004755.2. All detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands). We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: anti PIWIL1 antibody, anti HIWI antibody, anti PIW
• Research Area: Developmental Biology, Epigenetics
• Note: For research use only
• Entrez: 9271

Immunohistochemical analysis of formalin-fixed and paraffin embedded Human Testis tissue (4ug/ml) using PIWI antibody