Goat anti-PIM2 Antibody

SKU: orb131688

Description

Goat polyclonal to PIM2

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Dilution Range: ELISA: 1:16000, WB: 0.5-1 μg/ml, IHC-P: 5 μg/ml
• Reactivity: Human
• Application Notes:
  In transfected HEK293 transiently expressing Human PIM2 (myc and DYKDDDDK tagged), a band of approx. 38kDa is observed. No bands are observed in mock-transfected HEK293 and the same band is observed using anti-myc antibody. Recommended concentration, 0.5-1 μg/ml.WB: In transfected HEK293 transiently expressing Human PIM2 (myc and DYKDDDDK tagged), a band of approx. 38kDa is observed. No bands are observed in mock-transfected HEK293 and the same band is observed using anti-myc antibody. Recommended concentration, 0.5-1 μg/ml.

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: PIM2
• Protein Sequence: QTPAEDVPLNPSK
• Molecular Weight: 34.2
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

PIM2; pim-2 oncogene; pim-2h; proto-oncogene Pim-2 (serine threonine kinase); serine/threonine protein kinase pim-2; serine/threonine-protein kinase pim-2

Western blot analysis of HEK293 lysate (10ug protein in RIPA buffer) overexpressing Human using PIM2 antibody

orb131688 Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

orb131688 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic and membrane staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

orb131688 (5µg/ml) staining of paraffin embedded Human Testis. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

orb131688 (1µg/ml) staining of HepG2 (A) and K562 (B) cell lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.

Quick Database Links

Gene Symbol

PIM2

NCBI Gene ID

11040