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PHR1 Antibody

SKU: orb20063

Description

Goat polyclonal antibody to PLEKHB1

Research Area

Developmental Biology

Images & Validation

Tested ApplicationsELISA, WB
Dilution RangeELISA: 1:16000, WB: 1-3 μg/ml
ReactivityRat
Predicted ReactivityBovine, Human, Mouse
Application Notes
ELISA: Peptide ELISA: antibody detection limit dilution 1:16000.WB: Approx. 25kDa band observed in Rat Brain lysates (calculated MW of 25.2kDa according to Rat NP_742030.1 (Evectin-1) and of 27.2kDa according to Human NP_067023.1). Recommended concentration: 1-3 μg/ml.

Key Properties

HostGoat
ClonalityPolyclonal
TargetPHR1
Protein SequencePPDSALESPFEE
Molecular Weight27.2; 23.2
PurificationPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesSupplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

anti PLEKHB1 antibody, anti pleckstrin homology domain containing, family B (evectins) member 1 antibody, anti KPL1 antibody, anti PHR1 antibody, anti PHRET1 antibody, anti PH domain containing protein in retina 1 antibody, anti PH domain containing, retinal 1 antibody, anti evectin-1 antibody

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PHR1 Antibody

orb20063 (1ug/ml) staining of Rat Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

NCBI Gene Details

No NCBI Gene data available

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Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol

PHR1 Antibody (orb20063)

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Available Sizes

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100 μg
$ 530.00
DispatchUsually dispatched within 5-10 working days
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