PHR1 Antibody

• Catalog Number: orb20063
• Category: Antibodies
• Description: Goat polyclonal antibody to PLEKHB1
• Target: PHR1
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Rat
• Predicted Reactivity: Bovine, Human, Mouse
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Protein Sequence: PPDSALESPFEE
• RRID: AB_10749152
• MW: 27.2; 23.2
• Tested applications: ELISA, WB
• Dilution range: ELISA: 1:16000, WB: 1-3 μg/ml
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:16000.WB: Approx. 25kDa band observed in Rat Brain lysates (calculated MW of 25.2kDa according to Rat NP_742030.1 (Evectin-1) and of 27.2kDa according to Human NP_067023.1). Recommended concentration: 1-3 μg/ml.
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: anti PLEKHB1 antibody, anti pleckstrin homology do
• Research Area: Developmental Biology
• Note: For research use only
• Entrez: 58473

orb20063 (1ug/ml) staining of Rat Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.