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| Catalog Number | orb19205 |
|---|---|
| Category | Antibodies |
| Description | Goat polyclonal antibody to PGRMC1 |
| Target | PGRMC1 / MPR |
| Clonality | Polyclonal |
| Species/Host | Goat |
| Conjugation | Unconjugated |
| Reactivity | Human, Mouse |
| Predicted Reactivity | Rat |
| Concentration | 0.1-0.3 μg/ml |
| Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Protein Sequence | EPKDESARKND |
| RRID | AB_10755906 |
| MW | 21.7 |
| Tested applications | ELISA, IF, IHC, WB |
| Dilution range | ELISA: 1:4000, WB: 0.1-0.3 μg/ml, IHC-P: 5 μg/ml |
| Application notes | ELISA: Peptide ELISA: antibody detection limit dilution 1:4000.WB: Approx 26kDa band observed in Human Kidney, Liver and Testis lysates (calculated MW of 21.7kDa according to NP_006658.1). In transfected HEK293 transiently expressing Human PGRMC1 a band of approx. 26kDa is observed. This band is not observed in the non-transfected HEK293. The endogenous 20kDa protein is observed in both. Recommended concentration: 0.03-0.1 μg/ml. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Alternative names | anti PGRMC1 antibody, anti MPR antibody, anti prog Read more... |
| Research Area | Cardiovascular Disease, Epigenetics |
| Note | For research use only |
| Entrez | 10857 |

Western blot analysis of Human Liver lysate using PGRMC1 antibody

Western blot analysis of Human Kidney lysate using PGRMC1 antibody

Western blot analysis of Human Kidney (A), Liver (B) and Testes (C) lysate using PGRMC1 antibody
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