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Perilipin 1 (C Terminus) Antibody

SKU: orb19500

Description

Goat polyclonal antibody to PLIN

Images & Validation

Tested ApplicationsELISA, WB
Dilution RangeELISA: 1:128000, WB: 0.3-1 μg/ml
ReactivityHuman
Predicted ReactivityCanine, Rat
Application Notes
ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.WB: Approx 55-60kDa band observed in Human Adipose lysates (calculated MW of 56.0kDa according to NP_002657.2). Recommended concentration: 0.01-0.03 μg/ml.Experiment Notes: Immunofluorescence: Small lipid droplets preferentially stained in the periphery of differentiated 3T3-L1 adipocytes. Recommended concentration 1ug/ml.

Key Properties

HostGoat
ClonalityPolyclonal
TargetPerilipin 1 (C Terminus)
Protein SequenceEPILGRAQYSQLRKK
Molecular Weight56
PurificationPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesSupplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

anti Plin antibody, anti perilipin antibody, anti PERI antibody, anti lipid droplet-associated protein antibody

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Perilipin 1 (C Terminus) Antibody

Western blot analysis of human adipose lysate (0.01ug/ml) using Perilipin antibody

Perilipin 1 (C Terminus) Antibody

Immunofluorescence analysis of using PLIN antibody

Perilipin 1 (C Terminus) Antibody

Immunohistochemical staining of Human Colon using PLIN antibody

NCBI Gene Details

No NCBI Gene data available

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Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol

Perilipin 1 (C Terminus) Antibody (orb19500)

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