PDIA3/ERp57/GRP58 Antibody

SKU: orb18810

Description

Goat polyclonal antibody to PDIA3

Research Area

Epigenetics

Images & Validation

• Tested Applications: ELISA, WB
• Dilution Range: ELISA: 1:4000, WB: 0.1-0.3 μg/ml
• Reactivity: Human, Mouse
• Predicted Reactivity: Bovine, Rat
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:8000.WB: Approx 55kDa band observed in human hepatoblastoma HepG2 lysates (calculated MW of 56.8kDa according to NP_005304.3). Recommended concentration: 1-3 μg/ml.

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: PDIA3 / ERp57 / GRP58
• Protein Sequence: EEKPKKKKKAQEDL
• Molecular Weight: 56.8
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti PDIA3 antibody, anti GRP58 antibody, anti ERp57 antibody, anti protein disulfide isomerase family A, member 3 antibody, anti ERp60 antibody, anti ERp61 antibody, anti GRP57 antibody, anti HsT17083 antibody, anti P58 antibody, anti PI-PLC antibody, anti 58 kDa microsomal protein antibody, anti endoplasmic reticulum P58 antibody, anti glucose regulated protein, 58kDa antibody, anti phospholipase C-alpha antibody, anti protein disulfide isomerase-associated 3 antibody, anti ER60 antibody, anti protein disulfide-isomerase A3 antibody

orb18810 staining (1µg/ml) of HepG2 lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Quick Database Links

Gene Symbol

PDIA3 / ERp57 / GRP58

NCBI Gene ID

2923