Goat anti-PDE4D Antibody

SKU: orb19141

Description

Goat polyclonal antibody to PDE4D

Research Area

Epigenetics

Images & Validation

• Tested Applications: EIA, ELISA, IHC, WB
• Dilution Range: ELISA: 1:128000, WB: 0.1-0.3 μg/ml, IHC-P: 5 μg/ml
• Reactivity: Human
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:128000.WB: Approx 70+80+90kDa bands observed in lysates of cell line A549. In transfected COS1 transiently expressing PDE4D1 and PDE4D3 bands of approx. 68kDa and 95kDa are observed. These bands are not observed in the untransfected COS1. In transfected HEK293 transiently expressing PDE4D2 multi bands from approx. 55kDa to 80kDa are observed. These bands are not observed in the non-transfected HEK293. Calculated MW of 91.1kDa according to PDE4D1 (NP_001098101.1), 76.5kDa according to PDE4D2 (NP_006194.2) and 84.7kDa according to PDE4D3 (NP_001159371.1). Recommended concentration: 0.1-0.3 μg/ml.

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: PDE4D
• Protein Sequence: QPEACVIDDRSPDT
• Molecular Weight: 66.5; 57.8; 76.5; 59.1; 84.4; 91.1; 86.7
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti PDE4D antibody, anti phosphodiesterase 4D, cAMP-specific (phosphodiesterase E3 dunce homolog, Drosophila) antibody, anti HGNC:8783 antibody, anti DPDE3 antibody, anti HSPDE4D antibody, anti PDE4DN2 antibody, anti STRK1 antibody, anti cAMP-specific phosphodiesterase 4D antibody, anti cAMP-specific phosphodiesterase PDE4D6 antibody, anti dunce-like phosphodiesterase E3 antibody, anti phosphodiesterase 4D, cAMP-specific (dunce (Drosophila)-homolog phosphodiesterase E3) antibody, anti DKFZp686M11213 antibody, anti FLJ97311 antibody, anti phosphodiesterase 4D antibody

Western blot analysis of COS cell lysates using PDE4D antibody

Immunohistochemical staining of Human Skeletal Muscle using PDE4D antibody

Immunohistochemical staining of Human Heart using PDE4D antibody

Western blot analysis of HEK293 lysate using PDE4D antibody

Quick Database Links

Gene Symbol

PDE4D

NCBI Gene ID

5144