Goat anti-PDE4D Antibody

• Catalog Number: orb19141
• Category: Antibodies
• Description: Goat polyclonal antibody to PDE4D
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: EIA, ELISA, IHC, WB
• Reactivity: Human
• Dilution range: ELISA: 1:128000, WB: 0.1-0.3 μg/ml, IHC-P: 5 μg/ml
• Conjugation: Unconjugated
• MW: 66.5; 57.8; 76.5; 59.1; 84.4; 91.1; 86.7
• Target: PDE4D
• Entrez: 5144
• Protein Sequence: QPEACVIDDRSPDT
• RRID: AB_10755761
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: anti PDE4D antibody, anti phosphodiesterase 4D, cA
• Note: For research use only
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:128000.WB: Approx 70+80+90kDa bands observed in lysates of cell line A549. In transfected COS1 transiently expressing PDE4D1 and PDE4D3 bands of approx. 68kDa and 95kDa are observed. These bands are not observed in the untransfected COS1. In transfected HEK293 transiently expressing PDE4D2 multi bands from approx. 55kDa to 80kDa are observed. These bands are not observed in the non-transfected HEK293. Calculated MW of 91.1kDa according to PDE4D1 (NP_001098101.1), 76.5kDa according to PDE4D2 (NP_006194.2) and 84.7kDa according to PDE4D3 (NP_001159371.1). Recommended concentration: 0.1-0.3 μg/ml.
• Expiration Date: 12 months from date of receipt.

Western blot analysis of COS cell lysates using PDE4D antibody

Immunohistochemical staining of Human Skeletal Muscle using PDE4D antibody

Immunohistochemical staining of Human Heart using PDE4D antibody

Western blot analysis of HEK293 lysate using PDE4D antibody