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Item 1 of 3
Goat anti-PCSK9 (C Terminus) Antibody
Catalog Number: orb18975
| Catalog Number | orb18975 |
|---|---|
| Category | Antibodies |
| Description | Goat polyclonal antibody to PCSK9 |
| Target | PCSK9 (C Terminus) |
| Species/Host | Goat |
| Conjugation | Unconjugated |
| Reactivity | Human |
| Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
| Protein Sequence | CRSRHLAQASQELQ |
| RRID | AB_10749943 |
| Tested applications | ELISA, IHC, WB |
| Dilution range | ELISA: 1:16000, WB: 0.3-1 μg/ml |
| Application notes | WB: Approx 75kDa band observed in Human Adipose lysates (calculated MW of 74.3kDa according to NP_777596.2). Recommended concentration: 0.3-1µg/ml. Primary incubation was 1 hour. Constructs and lysates were as described in Sun et al Hum Mol Genet. 2005 May 1;. IHC: Paraffin embedded Human Liver. Recommended concentration: 5-6µg/ml. |
| Alternative names | PCSK9; FH3; NARC1; HCHOLA3; proprotein convertase Read more... |
| Note | For research use only |
orb18975 staining (0.2ug/ml) of McA cell lysates: untransfected (1), transfected with wild type human pcsk9 (2), transfected with S127R human pcsk9 (3). Data kindly provided by Dr. X-M Sun, Hammersmith Hospital, London.
orb18975 (0.3µg/ml) staining of Human Adipose lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
orb18975 (4µg/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with Tris/EDTA buffer pH 9, HRP-staining. This data is from a previous batch.
