Goat anti-PCNA Antibody

• Catalog Number: orb12280
• Category: Antibodies
• Description: Goat polyclonal to PCNA.
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Reactivity: Bovine, Canine, Human, Mouse, Porcine, Rat
• Dilution range: ELISA: 1:128000, WB: 0.01-0.03 μg/ml
• Conjugation: Unconjugated
• MW: 28.8
• Target: PCNA
• Entrez: 5111
• Protein Sequence: NGNIKLSQTSNVD
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: anti PCNA antibody
• Note: For research use only
• Application notes: WB: Approx 30kDa band observed in lysates of cell line HeLa (calculated MW of 28.8kDa according to Human NP_002583.1). Approx.33kDa band observed in lysates of cell line NIH3T3 and in Mouse and Rat Testis and Pig Spleen lysates (calculated MW of 28.8kDa acco. IHC: Paraffin embedded Human Testis. Recommended concentration: 5-6µg/ml.. Notes: Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371.
• Expiration Date: 12 months from date of receipt.

Western blot analysis of HeLa lysate (35ug protein in RIPA buffer) using PCNA antibody

Western blot analysis of NIH3T3 (A), Mouse Testis (B) and Rat Testis (C) lysates (35ug protein in RIPA buffer) using PCNA antibody

Western blot analysis of staining of Pig Spleen lysate (35µg protein in RIPA buffer) using PCNA antibody.