PCNA Antibody

SKU: orb12280

Description

Goat polyclonal to PCNA.

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Dilution range: ELISA: 1:128000, WB: 0.01-0.03 μg/ml
• Reactivity: Human, Mouse, Porcine, Rat
• Predicted Reactivity: Bovine, Canine
• Application Notes:
  WB: Approx 30kDa band observed in lysates of cell line HeLa (calculated MW of 28.8kDa according to Human NP_002583.1). Approx.33kDa band observed in lysates of cell line NIH3T3 and in Mouse and Rat Testis and Pig Spleen lysates (calculated MW of 28.8kDa acco. IHC: Paraffin embedded Human Testis. Recommended concentration: 5-6µg/ml.. Notes: Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371.

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: PCNA
• Molecular Weight: 28.8
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Disclaimer: For research use only

Alternative Names

anti PCNA antibody

Western blot analysis of HeLa lysate (35ug protein in RIPA buffer) using PCNA antibody

Western blot analysis of NIH3T3 (A), Mouse Testis (B) and Rat Testis (C) lysates (35ug protein in RIPA buffer) using PCNA antibody

Western blot analysis of staining of Pig Spleen lysate (35µg protein in RIPA buffer) using PCNA antibody.

Quick Database Links

Gene Symbol

PCNA

NCBI Gene ID

5111