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Goat anti-PCK1 / PEPCKC (internal) Antibody

Catalog Number: orb19167

DispatchUsually dispatched within 5-10 working days
$ 500.00
Catalog Numberorb19167
CategoryAntibodies
DescriptionGoat polyclonal antibody to PCK1
TargetPCK1 / PEPCKC (internal)
ClonalityPolyclonal
Species/HostGoat
ConjugationUnconjugated
ReactivityBovine, Canine, Human, Mouse, Porcine, Rat
Buffer/PreservativesSupplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Protein SequenceHVNWFRKDKEGK
RRIDAB_10752574
MW69.2
Tested applicationsELISA, IHC
Dilution rangeELISA: 1:8000, WB: 0.3 μg/ml, IHC-P: 2 μg/ml
Application notesELISA: Peptide ELISA: antibody detection limit dilution 1:8000.IHC: In paraffin embedded Human Cerebral Cortex shows staining of cytoplasm in pyramidal cells. Recommended concentration, 2-4ug/ml.WB: Preliminary experiments gave bands at approx 70kDa, 45kDa and 28kDa in Human Adipose, Kidney and Liver lysates after 0.3 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the additional bands we observe given the calculated size of 69.2 kDa according to NP_002582.2. All three detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands). We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported
StorageAliquot and store at -20°C. Minimize freezing and thawing.
Alternative namesanti PCK1 antibody, anti phosphoenolpyruvate carbo
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NoteFor research use only
Entrez5105
Goat anti-PCK1 / PEPCKC (internal) Antibody

2 µg/ml staining of paraffin embedded Human Cerebral Cortex. Steamed antigen retrieval with citrate buffer pH6, AP-staining.

Goat anti-PCK1 / PEPCKC (internal) Antibody

2 µg/ml staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH6, AP-staining.

Goat anti-PCK1 / PEPCKC (internal) Antibody

2 µg/ml staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH6, AP-staining.