Goat anti-PCDH11Y / PCDH11X Antibody

SKU: orb20350

Description

Goat polyclonal antibody to PCDH11Y

Research Area

Epigenetics

Images & Validation

• Tested Applications: ELISA, IHC
• Dilution Range: ELISA: 1:8000, IHC-P: 6μg/ml
• Reactivity: Human
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:8000.WB: Preliminary experiments gave an approx 75kDa band in Human Brain (Amygdala, Hippocampus, Substantia nigra) lysates after 1 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 115kDa according to NP_116753.1 and of 113kDa according to NP_055337.1, the smallest reported isoforms. The 75kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: PCDH11Y / PCDH11X
• Protein Sequence: KYNWVTTPTTFK
• Molecular Weight: 115; 116; 147; 113; 113; 148
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti PCDH11Y antibody, anti protocadherin 11 Y-linked antibody, anti PCDH-PC antibody, anti PCDH11X antibody, anti PCDH22 antibody, anti PCDHY antibody, anti OTTHUMP00000033077 antibody, anti protocadherin 11 X-linked antibody, anti protocadherin 22 antibody, anti protocadherin Y antibody, anti RP13-138P15.1 antibody, anti PCDH-X antibody, anti PCDH11 antibody, anti PCDHX antibody, anti protocadherin 11X antibody, anti protocadherin X antibody, anti protocadherin-S antibody

Immunohistochemical staining of Human Cortex using PCDH11Y antibody.

Immunohistochemical staining of Human Cortex using PCDH11Y antibody.

Quick Database Links

Gene Symbol

PCDH11Y / PCDH11X

NCBI Gene ID

27328, 83259