Goat anti-PCDH11Y / PCDH11X Antibody

• Catalog Number: orb20350
• Category: Antibodies
• Description: Goat polyclonal antibody to PCDH11Y
• Target: PCDH11Y / PCDH11X
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Human
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Protein Sequence: KYNWVTTPTTFK
• RRID: AB_10747410
• MW: 115; 116; 147; 113; 113; 148
• Tested applications: ELISA, IHC
• Dilution range: ELISA: 1:8000, IHC-P: 6μg/ml
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:8000.WB: Preliminary experiments gave an approx 75kDa band in Human Brain (Amygdala, Hippocampus, Substantia nigra) lysates after 1 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 115kDa according to NP_116753.1 and of 113kDa according to NP_055337.1, the smallest reported isoforms. The 75kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: anti PCDH11Y antibody, anti protocadherin 11 Y-lin
• Research Area: Epigenetics
• Note: For research use only
• Entrez: 27328, 83259

Immunohistochemical staining of Human Cortex using PCDH11Y antibody.

Immunohistochemical staining of Human Cortex using PCDH11Y antibody.