PAX8 (N Terminus) Antibody

SKU: orb18707

Description

Goat polyclonal antibody to PAX8

Research Area

Developmental Biology, Epigenetics

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Dilution Range: ELISA: 1:128000, WB: 1-2 μg/ml, IHC-P: 5 μg/ml
• Reactivity: Human
• Predicted Reactivity: Mouse, Rat
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.IHC: In paraffin embedded Human Thyroid Gland shows nuclear staining in epithelial cells around follicles. Recommended concentration, 1-2ug/ml.A customer found this antibody unsuccessful in immunohistochemistry on Mouse tissues.WB: Approx 40kDa band observed in Human Liver lysates (calculated MW of xxkDa according to NP_039245.1). Recommended concentration: 1-3 μg/ml.

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: PAX8 (N Terminus)
• Protein Sequence: PHNSIRSGHGGLN
• Molecular Weight: 48.2; 41.7; 43.0; 34.9; 31.0
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti PAX8 antibody, anti paired box gene 8 antibody, anti paired domain gene 8 antibody, anti paired box 8 antibody

Immunohistochemical staining of human Thyroid Gland using PAX8 antibody

Western blot analysis of HEK293 lysate using PAX8 antibody

Quick Database Links

Gene Symbol

PAX8 (N Terminus)

NCBI Gene ID

7849