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Catalog Number | orb18763 |
---|---|
Category | Antibodies |
Description | Goat polyclonal antibody to PAX3 |
Species/Host | Goat |
Clonality | Polyclonal |
Tested applications | ELISA, IHC, WB |
Reactivity | Bovine, Human, Rat |
Dilution range | ELISA: 1:32000, WB: 0.3-1 μg/ml, IHC-P: 4.5 μg/ml |
Conjugation | Unconjugated |
MW | 53.0; 24.1; 22.7; 53.5; 56.0; 44.8; 45.2; 53.3 |
Target | PAX3 |
Entrez | 5077 |
Protein Sequence | TTLAGAVPRMM |
RRID | AB_10755589 |
Storage | Aliquot and store at -20°C. Minimize freezing and thawing. |
Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
Alternative names | anti PAX3 antibody, anti WS1 antibody, anti CDHS a Read more... |
Note | For research use only |
Application notes | ELISA: Peptide ELISA: antibody detection limit dilution 1:64000.IHC: In paraffin embedded Human Oesophagus shows nuclear staining of cells in the Squamous Epithelium. Recommended concentration, 3ug/ml.WB: Preliminary experiments gave an approx 30-35kDa band in Human Kidney lysates at 1 μg/ml. Please note that currently we cannot find an explanation in the literature for the band we observe given the predicted sizes of approx. 24-58kDda according to NP_000429.2; NP_039230.1; NP_852122;.1 NP_852123.1; NP_852124.1; NP_852125.1; NP_852126.1. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of Human Duodenum lysate using PAX3 antibody
Immunohistochemical staining of Human Esophagus using PAX3 antibody
Immunohistochemical staining of Human Esophagus using PAX3 antibody
ELISA, IHC, WB | |
Bovine, Human, Mouse, Rat | |
Goat | |
Polyclonal | |
Unconjugated |
ELISA, WB | |
Bovine, Canine, Human, Mouse, Porcine, Rat | |
Goat | |
Polyclonal | |
Unconjugated |