PAX3 Antibody

SKU: orb18763

Description

Goat polyclonal antibody to PAX3

Research Area

Cancer, Developmental Biology, Epigenetics, Stem Cells

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Dilution Range: ELISA: 1:32000, WB: 0.3-1 μg/ml, IHC-P: 4.5 μg/ml
• Reactivity: Human
• Predicted Reactivity: Bovine, Rat
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:64000.IHC: In paraffin embedded Human Oesophagus shows nuclear staining of cells in the Squamous Epithelium. Recommended concentration, 3ug/ml.WB: Preliminary experiments gave an approx 30-35kDa band in Human Kidney lysates at 1 μg/ml. Please note that currently we cannot find an explanation in the literature for the band we observe given the predicted sizes of approx. 24-58kDda according to NP_000429.2; NP_039230.1; NP_852122;.1 NP_852123.1; NP_852124.1; NP_852125.1; NP_852126.1. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: PAX3
• Protein Sequence: TTLAGAVPRMM
• Molecular Weight: 53.0; 24.1; 22.7; 53.5; 56.0; 44.8; 45.2; 53.3
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti PAX3 antibody, anti WS1 antibody, anti CDHS antibody, anti HUP2 antibody, anti paired box gene 3 (Waardenburg syndrome 1) antibody, anti paired domain gene 3 antibody, anti paired box homeotic gene 3 (Waardenburg syndrome 1) antibody, anti paired box 3 antibody, anti MGC120381 antibody, anti MGC120382 antibody, anti MGC120383 antibody, anti MGC120384 antibody, anti MGC134778 antibody, anti paired box homeotic gene 3 antibody, anti paired domain gene HuP2 antibody

Western blot analysis of Human Duodenum lysate using PAX3 antibody

Immunohistochemical staining of Human Esophagus using PAX3 antibody

Immunohistochemical staining of Human Esophagus using PAX3 antibody

Quick Database Links

Gene Symbol

PAX3

NCBI Gene ID

5077