Goat anti-PARK7 / DJ-1 Antibody

SKU: orb18461

Description

Goat polyclonal antibody to PARK7

Images & Validation

• Tested Applications: ELISA, IF, IHC, WB
• Dilution range: ELISA: 1:128000, WB: 0.05-0.1 μg/ml, IHC-P: 5 μg/ml
• Reactivity: Human, Mouse, Rat
• Application Notes:
  Approx 20kDa band seen in Jurkat lysate [Predicted MW of approx. 20kDa according to NP_009193]. Recommended for use at 0.5-1 μg/ml.ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: PARK7 / DJ-1
• Molecular Weight: 19.9
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Disclaimer: For research use only

Alternative Names

PARK7; DJ1; DJ-1; Parkinson disease (autosomal recessive, early onset) 7; oncogene DJ1; CTA-215D11.1; FLJ27376; FLJ34360; FLJ92274; OTTHUMP00000001350; OTTHUMP00000001351; Parkinson disease protein 7; protein DJ-1

orb18461 staining (1ug/ml) of Jurkat lysate (RIPA buffer, 30ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

orb18461 (0.001ug/ml) staining of HeLa (A) and Jurkat (B) lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

orb18461 (0.001ug/ml) staining of Human Cerebellum (A), Human Frontal Cortex (B), Human Hippocampus (C) Mouse Fetal Brain (D), Mouse Brain (E) and Rat Brain (F) lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemilumines

Immunofluorescence staining of MCF7 (A) and Hela (B) cells with 5ug/ml orb18461 antibody. Detected with Rabbit anti-goat IgG-Alexafluor488 antibody at 1:1000. Nuclei Counterstained with DAPI.

Quick Database Links

Gene Symbol

PARK7 / DJ-1

NCBI Gene ID

11315