Goat anti-PAR6alpha / PARD6A Antibody

• Catalog Number: orb18626
• Category: Antibodies
• Description: Goat polyclonal antibody to PARD6A
• Target: PAR6alpha / PARD6A
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Human
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Protein Sequence: GSRIRGDGSGFSL
• RRID: AB_10748176
• MW: 37.4; 37.3
• Tested applications: ELISA, FC, IHC, WB
• Dilution range: ELISA: 1:8000, WB: 1-3 μg/ml, IHC-P: 5-10 μg/ml
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:8000.IHC: In paraffin embedded Human Pancreas, shows strong cytoplasmic staining in rare cells of intralobular ducts. Recommended concentration, 10ug/ml. Also tested in paraffin embedded Human Heart where it shows speckled staining on myocardial fibres in transverWB: Preliminary experiments gave no signal but low background in Human Brain and Pancreas extracts at up to 1 μg/ml.
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: anti PARD6A antibody, anti PAR6alpha antibody, ant
• Note: For research use only
• Entrez: 50855

orb18626 (10ug/ml) staining of paraffin embedded Human Pancreas. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining.

orb18626 (5ug/ml) staining of paraffin embedded Human Heart. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Western blot analysis of Jurkat (Lane 1) and U251 (Lane 2) cell lysate using PARD6A antibody

Flow cytometric analysis of paraformaldehyde fixed Jurkat cells using PARD6A antibody