Pancreatic Polypeptide/PPY Antibody

SKU: orb19027

Description

Goat polyclonal antibody to PPY

Images & Validation

• Tested Applications: ELISA, FC, IF, IHC, WB
• Dilution range: ELISA: 1:32000, IHC-P: 5 μg/ml
• Reactivity: Human, Rat
• Predicted Reactivity: Canine, Mouse
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.IHC: In paraffin embedded human pancreas, shows strong cytoplasmic staining of rare cells located at the periphery of islets of Langerhans. Recommended concentration, 3ug/ml.WB: Preliminary experiments gave an approx 35-38kDa band in human pancreas lysates after 1 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 10.5kDa according to NP_002713.1. The 38kDa band was successfully blocked by incubation with the immunizing peptide.

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: Pancreatic Polypeptide / PPY
• Molecular Weight: 10.5; 11.2
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Disclaimer: For research use only

Alternative Names

anti PPY antibody, anti pancreatic polypeptide antibody, anti HGNC:9327 antibody, anti PNP antibody, anti pancreatic polypeptide Y antibody

Immunohistochemical staining of Human Pancreas using PPY antibody

Immunohistochemical staining of Human Intestine using PPY antibody

Immunohistochemical staining of Human Pancreas using PPY antibody

Quick Database Links

Gene Symbol

Pancreatic Polypeptide / PPY

NCBI Gene ID

5539