PALS/MPP5 Antibody

SKU: orb18856

Description

Goat polyclonal to MPP5

Research Area

Epigenetics

Images & Validation

• Tested Applications: ELISA, FC, IF, IHC
• Dilution Range: ELISA: 1:32000, WB: 1-3 μg/ml, IHC-P: 5 μg/ml
• Reactivity: Human
• Predicted Reactivity: Bovine, Canine, Porcine
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.IHC: In paraffin embedded Human Skeletal Muscle shows strong alternating staning of muscle fibres in longitudinal section. Recommended concentration, 5-10ug/ml.WB: Approx 75kDa band observed in Human Brain lysate (calculated MW of 77.3kDa according to NP_071919). Recommended concentration: 1-3 μg/ml.

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: PALS / MPP5
• Protein Sequence: NKASPPFPLISNAQ
• Molecular Weight: 77.3; 73.4
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti MPP5 antibody, anti PALS1 antibody, anti FLJ12615 antibody, anti membrane protein, palmitoylated 5 (MAGUK p55 subfamily member 5) antibody, anti stardust (Drosophila) antibody, anti MAGUK p55 subfamily member 5 antibody, anti membrane protein, palmitoylated 5 antibody, anti stardust antibody

orb18856 (1ug/ml) staining of Human Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

orb18856 (5ug/ml) staining of paraffin embedded Human Skeletal Muscle. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Immunohistochemical staining of Human Small Intestine using MPP5 antibody

Immunohistochemical staining of Human Kidney using MPP5 antibody

Quick Database Links

Gene Symbol

PALS / MPP5

NCBI Gene ID

64398