Goat anti-PALS / MPP5 Antibody

• Catalog Number: orb18856
• Category: Antibodies
• Description: Goat polyclonal to MPP5
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, FC, IF, IHC
• Reactivity: Bovine, Canine, Human, Porcine
• Dilution range: ELISA: 1:32000, WB: 1-3 μg/ml, IHC-P: 5 μg/ml
• Conjugation: Unconjugated
• MW: 77.3; 73.4
• Target: PALS / MPP5
• Entrez: 64398
• Protein Sequence: NKASPPFPLISNAQ
• RRID: AB_10749781
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: anti MPP5 antibody, anti PALS1 antibody, anti FLJ1
• Note: For research use only
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.IHC: In paraffin embedded Human Skeletal Muscle shows strong alternating staning of muscle fibres in longitudinal section. Recommended concentration, 5-10ug/ml.WB: Approx 75kDa band observed in Human Brain lysate (calculated MW of 77.3kDa according to NP_071919). Recommended concentration: 1-3 μg/ml.
• Expiration Date: 12 months from date of receipt.

orb18856 (1ug/ml) staining of Human Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

orb18856 (5ug/ml) staining of paraffin embedded Human Skeletal Muscle. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Immunohistochemical staining of Human Small Intestine using MPP5 antibody

Immunohistochemical staining of Human Kidney using MPP5 antibody