Palladin (aa895-907) Antibody

SKU: orb20083

Description

Goat polyclonal antibody to PALLD

Research Area

Epigenetics

Images & Validation

• Tested Applications: ELISA, WB
• Dilution Range: ELISA: 1:2000, WB: 0.1-0.3 μg/ml
• Reactivity: Human
• Predicted Reactivity: Canine, Mouse, Rat
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:2000.WB: Approx. 125kDa band observed in Human Umbillical Cord lysates (calculated MW of122kDa according to NP_057165.3). Recommended concentration: 0.1-0.3 μg/ml. An additional band of 55kDa was consistently observed, however this band was not blocked by the immunizing peptide and it is therefore a non-specific signal. We call for caution when used for other assays than Western blot.

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: Palladin (aa895-907)
• Protein Sequence: RDSGDENEPIQER
• Molecular Weight: 122
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti PALLD antibody, anti palladin, cytoskeletal associated protein antibody, anti CGI-151 antibody, anti FLJ22190 antibody, anti FLJ38193 antibody, anti FLJ39139 antibody, anti KIAA0992 antibody, anti PNCA1 antibody, anti SIH002 antibody, anti palladin antibody, anti sarcoma antigen NY-SAR-77 antibody

orb20083 (0.1ug/ml) staining of Human Umbillical Cord lysate (35ug protein in RIPA buffer) with (B) and without (A) blocking with the immunising peptide. Primary incubation was 1 hour. Detected by chemiluminescence.

Quick Database Links

Gene Symbol

Palladin (aa895-907)

NCBI Gene ID

23022