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Item 1 of 5
Goat anti-P2RX7 / P2X7 receptor Antibody
Catalog Number: orb20291
| Catalog Number | orb20291 |
|---|---|
| Category | Antibodies |
| Description | Goat polyclonal antibody to P2RX7 |
| Species/Host | Goat |
| Clonality | Polyclonal |
| Tested applications | ELISA, FC, IF, IHC, WB |
| Reactivity | Canine, Human, Mouse, Rat |
| Dilution range | ELISA: 1:16000, WB: 1-3 μg/ml, IHC-P: 3.75 μg/ml |
| Conjugation | Unconjugated |
| MW | 68.6 |
| Target | P2RX7 / P2X7 receptor |
| Entrez | 5027 |
| Protein Sequence | YETNKVTRIQSMNY |
| RRID | AB_10753748 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
| Alternative names | anti P2RX7 antibody, anti purinergic receptor P2X, Read more... |
| Note | For research use only |
| Application notes | ELISA: Peptide ELISA: antibody detection limit dilution 1:16000.WB: Approx 37kDa and 70kDa band observed in Human Brain (Frontal Cortex) lysates (calculated MW of 68.6kDa according to NP_002553.2). Recommended concentration: 1-3 μg/ml. |
| Expiration Date | 12 months from date of receipt. |
Immunohistochemical staining of Human Kidney using P2RX7 antibody
3.75 µg/mL staining of paraffin embedded Human Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
0.3 µg/mL staining of A431 (A) and HeLa (B) cell lysate (35 µg protein in RIPA buffer). Detected by chemiluminescence.
Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1 hr (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL), showing cytoplasmic and plasma membrane staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL).
Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1 hr (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (1 µg/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
