P27KIP1/CDKN1B (internal) Antibody

SKU: orb19180

Description

Goat polyclonal antibody to CDKN1B

Research Area

Cancer Biology

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Dilution Range: ELISA: 1:32000, WB: 0.1-0.3 μg/ml, IHC-P: 5 μg/ml
• Reactivity: Human
• Predicted Reactivity: Bovine, Canine, Feline, Mouse, Porcine, Rat
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.WB: Approx 26kDa band observed in lysates of Human Prostate and of Hepatoblastoma cell line HepG2 (calculated MW of 22.1kDa according to NP_004055.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Xia et al, Proc Natl Acad Sci U S A. 2005 Sep 27;102(39):14028-33; PMID: 16169901). Recommended concentration: 0.1-0.3 μg/ml.

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Key Properties

• Clonality: Polyclonal
• Target: P27KIP1 / CDKN1B (internal)
• Protein Sequence: KRPATDDSSTQNK
• Molecular Weight: 22.1
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti CDKN1B antibody, anti KIP1 antibody, anti cyclin-dependent kinase inhibitor 1B (p27, Kip1) antibody, anti CDKN4 antibody, anti P27KIP1 antibody, anti cyclin-dependent kinase inhibitor 1B antibody

Western blot analysis of human Prostate lysate using CDKN1B antibody

Immunohistochemical staining of Human Prostate using CDKN1B antibody

Immunohistochemical staining of Human Spleen Tissue of CDKN1B antibody.

Quick Database Links

Gene Symbol

P27KIP1 / CDKN1B (internal)

NCBI Gene ID

1027