Osteopontin/SPP1 Antibody

SKU: orb19047

Description

Goat polyclonal antibody to Osteopontin

Images & Validation

• Tested Applications: ELISA, WB
• Dilution range: ELISA: 1:32000, WB: 0.1-0.3 μg/ml
• Reactivity: Human
• Predicted Reactivity: Canine, Mouse, Rat
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:64000.WB: Approx 30kDa band observed in lysates of cell lines Jurkat and MOLT4. This band corresponds to the cleavage product from the 60kDa glycoprotein (calculated MW of 33.8kDa according to NP_000573.1). Recommended concentration: 1-3 μg/ml.

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: Osteopontin / SPP1
• Molecular Weight: 35.4; 33.8; 32.4
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Disclaimer: For research use only

Alternative Names

anti-BNSP antibody, anti-Bone sialoprotein 1 antibody, anti-BSP I antibody, anti-BSPI antibody, anti-ETA1 antibody, anti-Nephropontin antibody, anti-OPN antibody, anti-Osteopontin antibody, anti-Secreted phosphoprotein 1 antibody, anti-SPP 1 antibody, anti-SPP-1 antibody, anti-SPP1 antibody, anti-Uropontin antibody

orb19047 (0.5µg/ml) staining of MOLT4 lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Quick Database Links

Gene Symbol

Osteopontin / SPP1

NCBI Gene ID

6696