OGT Antibody

SKU: orb19441

Description

Goat polyclonal antibody to OGT

Images & Validation

• Tested Applications: ELISA, FC, IF, IHC, WB
• Dilution range: ELISA: 1:64000, WB: 0.05-0.2 μg/ml, IHC-P: 5 μg/ml
• Reactivity: Human, Rat
• Predicted Reactivity: Bovine, Canine, Mouse
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:64000.WB: Approx 110kDa band observed in Pancreas lysates from Rat and Mouse (calculated MW of 117kDa according to NP_858058.1. Recommended concentration: 0.05-0.2 μg/ml. An additional band of unknown identity was also consistently observed at 60kDa. This band was successfully blocked by incubation with the immunising peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: OGT
• Molecular Weight: 117, 116
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Disclaimer: For research use only

Alternative Names

anti OGT antibody, anti O-linked N-acetylglucosamine (GlcNAc) transferase (UDP-N-acetylglucosamine:polypeptide-N-acetylglucosaminyl transferase) antibody, anti FLJ23071 antibody, anti HRNT1 antibody, anti MGC22921 antibody, anti O-GLCNAC antibody, anti O-GlcNAc transferase p110 subunit O-linked GlcNAc transferase antibody, anti uridinediphospho-N-acetylglucosamine:polypeptide beta-N-acetylglucosaminyl transferase antibody

Western blot analysis of Rat Pancreas lysate using OGT antibody

Immunohistochemical staining of Human Cortex using OGT antibody

Immunofluorescence analysis of HeLa cells using OGT antibody

Immunofluorescence analysis of U2OS cells using OGT antibody

Flow cytometric analysis of HEK293 cells using OGT antibody

Immunofluorescence analysis of U251 cells using OGT antibody

Quick Database Links

Gene Symbol

OGT

NCBI Gene ID

8473