Goat anti-Nur77 / TR3 (isoform a) Antibody

• Catalog Number: orb19369
• Category: Antibodies
• Description: Goat polyclonal antibody to NR4A1
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Reactivity: Canine, Human, Mouse, Rat
• Concentration: 1-3 μg/ml
• Dilution range: ELISA: 1:32000, WB: 1-3 μg/ml, IHC-P: 5 μg/ml
• Conjugation: Unconjugated
• MW: 64.5
• Target: Nur77 / TR3 (isoform a)
• Entrez: 3164
• Protein Sequence: GRLPSKPKQPPDAS
• RRID: AB_10748651
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: anti NR4A1 antibody, anti NUR77 antibody, anti nuc
• Note: For research use only
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.WB: Approx 60kDa band observed in Human Thyroid gland lysates (calculated MW of 64.5kDa according to NP_002126.2 and NP_775180.1). Recommended concentration: 1-3 μg/ml.
• Expiration Date: 12 months from date of receipt.

orb19369 (2ug/ml) staining of Human Thyroid lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

orb19369 (5µg/ml) staining of paraffin embedded Human Breast. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.