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Nur77/TR3 (isoform a) Antibody

SKU: orb19369

Description

Goat polyclonal antibody to NR4A1

Research Area

Immunology & Inflammation

Images & Validation

Tested ApplicationsELISA, IHC, WB
Dilution RangeELISA: 1:32000, WB: 1-3 μg/ml, IHC-P: 5 μg/ml
ReactivityHuman
Predicted ReactivityCanine, Mouse, Rat
Application Notes
ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.WB: Approx 60kDa band observed in Human Thyroid gland lysates (calculated MW of 64.5kDa according to NP_002126.2 and NP_775180.1). Recommended concentration: 1-3 μg/ml.

Key Properties

HostGoat
ClonalityPolyclonal
TargetNur77 / TR3 (isoform a)
Protein SequenceGRLPSKPKQPPDAS
Molecular Weight64.5
PurificationPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesSupplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration1-3 μg/ml
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

anti NR4A1 antibody, anti NUR77 antibody, anti nuclear receptor subfamily 4, group A, member 1 antibody, anti GFRP1 antibody, anti HMR antibody, anti MGC9485 antibody, anti N10 antibody, anti NAK-1 antibody, anti NGFIB antibody, anti NP10 antibody, anti TR3 antibody, anti TR3 orphan receptor antibody, anti early response protein NAK1 antibody, anti growth factor-inducible nuclear protein N10 antibody, anti hormone receptor antibody, anti orphan nuclear receptor HMR antibody, anti steroid receptor TR3 antibody
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Quality Guarantee

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Nur77/TR3 (isoform a) Antibody

orb19369 (2ug/ml) staining of Human Thyroid lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Nur77/TR3 (isoform a) Antibody

orb19369 (5µg/ml) staining of paraffin embedded Human Breast. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

NCBI Gene Details

No NCBI Gene data available

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Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
IHC
Immunohistochemistry
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol

Nur77/TR3 (isoform a) Antibody (orb19369)

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Available Sizes

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100 μg
$ 530.00
DispatchUsually dispatched within 5-10 working days
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