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NUMB Antibody

SKU: orb18456

Description

Goat polyclonal antibody to NUMB

Research Area

Neuroscience

Images & Validation

Tested ApplicationsELISA, FC, IF, IHC
Dilution RangeELISA: 1:128000, WB: 1-2 μg/ml, IHC-P: 5 μg/ml
ReactivityHuman, Mouse
Predicted ReactivityBovine, Canine
Application Notes
ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.WB: Approx 80kDa band observed in Mouse Brain lysates (calculated MW of 70.8kDa according to NP_001005743.1). Recommended concentration: 0.03-0.1 μg/ml.

Key Properties

ClonalityPolyclonal
TargetNUMB
Protein SequencePFSSDLQKTFEIEL
Molecular Weight70.8; 65.9; 69.4; 64.5
PurificationPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesSupplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

anti NUMB antibody, anti numb homolog (Drosophila) antibody, anti S171 antibody, anti numb homolog antibody

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NCBI Gene Details

No NCBI Gene data available

Protocol Information

IHC
Immunohistochemistry
View Protocol
FC
Flow Cytometry
View Protocol
IF
Immunofluorescence
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol

Available Sizes

Select a size below

100 μg
$ 560.00
Choose Conjugation or Carrier Free Version
Free Secondary Antibody (20 ul)0/0

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DispatchUsually dispatched within 5-10 working days
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