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Catalog Number | orb19888 |
---|---|
Category | Antibodies |
Description | Goat polyclonal antibody to NRAS |
Species/Host | Goat |
Clonality | Polyclonal |
Tested applications | ELISA, WB |
Reactivity | Human, Mouse, Rat |
Dilution range | ELISA: 1:64000, WB: 0.3-1μg/ml |
Conjugation | Unconjugated |
MW | 21.2 |
Target | NRAS |
Entrez | 4893 |
Protein Sequence | RMKKLNSSDDGTQ |
RRID | AB_10753722 |
Storage | Aliquot and store at -20°C. Minimize freezing and thawing. |
Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
Alternative names | anti NRAS antibody, anti neuroblastoma RAS viral ( Read more... |
Note | For research use only |
Application notes | ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.IHC: In paraffin embedded Human Small Intestine shows staining of cytoplasm and membrane of enterocytes. Recommended concentration, 2-3ug/ml.WB: Approx. 21kDa band observed in Human lysates of cell line A431 (calculated MW of 21.2kDa according to NP_002515.1). In transfected HEK293 transiently expressing NRAS bands of approx. 21kDa and 25kDa are observed. These bands are not observed in the non-transfected HEK293. Recommended concentration: 0.01-0.03 μg/ml. |
Expiration Date | 12 months from date of receipt. |
Immunohistochemical staining of Human Small Intestine using NRAS antibody
Western blot analysis of MCF7 cell lysate using NRAS antibody.