NR5A2/LRH1 Antibody

SKU: orb167711

Description

Goat polyclonal antibody to NR5A2

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Dilution range: Peptide ELISA: antibody detection limit dilution 1:128000. Western blot: Approx 55kDa band observed in nuclear lysates of cell lines HeLa and HepG2, and approx 60kDa band observed in Pig Ovary lysates (calculated MW of 56.5kDa according to Human NP_003813.1 and Pig AFD98842.1). Recommended concentration: 0.3-1µg/ml. Primary incubation was 1 hour. Preliminary testing was unsuccessful on Mouse Liver and NIH-3T3 cell line for this particular batch. Immunohistochemistry: This antibody has been successfully used in IHC on Mouse.
• Reactivity: Human, Porcine
• Predicted Reactivity: Bovine, Canine, Mouse, Rat

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: NR5A2 / LRH1
• Molecular Weight: 61.3; 56.5
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Disclaimer: For research use only

Alternative Names

anti NR5A2 antibody, anti Nuclear receptor subfamily 5 group A member 2 antibody, anti Nuclear receptor subfamily 5 group A member 2 antibody, anti B1-binding factor antibody, anti B1F antibody, anti B1F2 antibody, anti CPF antibody, anti CYP7A promoter-binding factor antibody, anti FTF antibody, anti FTZ F1 antibody, anti FTZ F1beta antibody, anti FTZ-F1 antibody, anti FTZ-F1beta antibody, anti hB1F 2 antibody, anti HB1F antibody, anti hB1F-2 antibody, anti Hepatocytic transcription factor antibody, anti Liver receptor homolog 1 antibody, anti LRH-1 antibody, anti LRH1 antibody, anti Nr5a2 antibody, anti NR5A2_HUMAN antibody, anti Nuclear receptor subfamily 5 group A member 2 antibody

0.3 µg/ml staining of Pig Ovary lysate (35 µg protein in RIPA buffer). Detected by chemiluminescence.

1 µg/ml staining of HepG2 nuclear lysate (35 µg protein in RIPA buffer). Detected by chemiluminescence.

Quick Database Links

Gene Symbol

NR5A2 / LRH1

NCBI Gene ID

2494