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Npc1(mouse) Antibody

SKU: orb20578

Description

Goat polyclonal antibody to NPC1

Research Area

Epigenetics, Infectious Diseases

Images & Validation

Tested ApplicationsELISA
Dilution RangeELISA: 1:16000, WB: 1 μg/ml
Predicted ReactivityMouse
Application Notes
ELISA: Peptide ELISA: antibody detection limit dilution 1:16000.WB: Preliminary experiments gave an approx 36kDa band in lysates of cell lines NIH3T3 and NSO after 1 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 142kDa according to NP_032746.2. The 36kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported

Key Properties

HostGoat
ClonalityPolyclonal
TargetNpc1(mouse)
Protein SequenceTYHTILKTSADYTD
Molecular Weight142
PurificationPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesSupplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

anti-Niemann Pick C1 protein precursor antibody, anti-Niemann Pick disease, type C1 antibody, anti-Niemann-Pick C1 protein antibody, anti-NPC antibody, anti-NPC1 antibody, anti-NPC-1 antibody, anti-NPC 1 antibody

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Protocol Information

ELISA
Enzyme-linked Immunosorbent Assay (EIA)
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Npc1(mouse) Antibody (orb20578)

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100 μg
$ 330.00
DispatchUsually dispatched within 5-10 working days
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