NOX5 Antibody

• Catalog Number: orb125115
• Category: Antibodies
• Description: Goat polyclonal antibody to NOX5
• Target: NOX5
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Human
• Predicted Reactivity: Bovine, Canine
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Protein Sequence: EWHPFTISSAPEQKD
• MW: 86.4; 83.8; 83.1
• Tested applications: ELISA, WB
• Dilution range: ELISA: 1:8000, WB: 0.5-2 μg/ml, IHC-P: 10ug/ml
• Application notes: WB: Approx 90kDa band observed in Human Testis lysates (calculated MW of 86.4kDa according to NP_078781.3). Recommended concentration: 0.5-2µg/ml. Primary incubation 1 hour at room temperature.. Notes: Immunofluorescence: Strong expression of the protein seen in the plasma membrane and cytoplasm of HeLa and MCF7 cells. Recommended concentration:10ug/ml.
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: anti NOX5 antibody, anti NADPH oxidase, EF-hand ca
• Note: For research use only
• Entrez: 79400

1 µg/ml staining of HeLa (A) and Caco-2 (B) cell lysate (35 µg protein in RIPA buffer). Detected by chemiluminescence.

1.5 µg/ml staining of Human Adrenal Gland lysate (35 µg protein in RIPA buffer). Detected by chemiluminescence.