NOTCH4 (aa1261-1273) Antibody

• Catalog Number: orb125146
• Category: Antibodies
• Description: Goat polyclonal antibody to NOTCH4
• Target: NOTCH4 (aa1261-1273)
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Human, Mouse
• Predicted Reactivity: Bovine, Canine, Porcine, Rat
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Protein Sequence: DHFHNGHCEKGCNN
• MW: 210
• Tested applications: ELISA, IF
• Dilution range: ELISA: 1:8000, WB: 1 μg/ml, IHC-P: 10 μg/ml
• Application notes: WB: Preliminary experiments gave an approx 27kDa band in all human, mouse and rat tissue and cell line lysates tested after 1µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the. Notes: Immunofluorescence: Strong expression of the protein seen in the nuclei and membranes of HeLa cells, and in the membranes of NIH3T3 cells. Recommended concentration: 10µg/ml.
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: anti NOTCH4 antibody, anti DAQB-143M3.4 antibody,
• Research Area: Cardiovascular Disease, Neuroscience
• Note: For research use only
• Entrez: 4855

Immunofluorescence analysis of HeLa cells using NOTCH4 antibody

Immunofluorescence analysis of NIH3T3 cells using NOTCH4 antibody