NONO/p54NRB Antibody

SKU: orb19244

Description

Goat polyclonal antibody to NONO

Research Area

Cancer, DNA Damage, Epigenetics, Immunology

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Dilution Range: ELISA: 1:16000, WB: 0.1-0.3 μg/ml, IHC-P: 3.75 μg/ml
• Reactivity: Human, Mouse
• Predicted Reactivity: Canine, Rat
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:16000.WB: Approx 60kDa band observed in Mouse Brain lysates (calculated MW of 54.2kDa according to Human NP_031389.3 and 54.5kDa according to Mouse NP_075633.2). Recommended concentration: 0.1-0.3 μg/ml. IHC-P : Paraffin embedded Human Spleen and Testis. Recommended concentration: 3.75μg/ml.

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: NONO / p54NRB
• Protein Sequence: NRAAPGAEFAPNK
• Molecular Weight: 54.2
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti NONO antibody, anti P54NRB antibody, anti non-POU domain containing, octamer-binding antibody, anti HGNC:7871 antibody, anti NMT55 antibody, anti NRB54 antibody, anti P54 antibody, anti Nuclear RNA-binding protein, 54-kD antibody, anti non-POU-domain-containing, octamer-binding antibody, anti non-Pou domain-containing octamer (ATGCAAAT) binding protein antibody

Western blot analysis of Mouse Brain lysate using NONO antibody

Immunohistochemical staining of Human Spleen using NONO antibody

Immunohistochemical staining of Human Testis using NONO antibody

Quick Database Links

Gene Symbol

NONO / p54NRB

NCBI Gene ID

4841