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Nogo 66 receptor Antibody

SKU: orb19023

Description

Goat polyclonal antibody to RTN4R

Images & Validation

Tested ApplicationsELISA, WB
Dilution rangeELISA: 1:16000, WB: 0.3-1 μg/ml
ReactivityHuman
Predicted ReactivityCanine, Mouse, Porcine, Rat
Application Notes
ELISA: Peptide ELISA: antibody detection limit dilution 1:16000.WB: Experiments gave bands at approx 90-100kDa and 37+40+45kDa in Human brain lysate after 0.3 μg/ml antibody staining. These bands were successfully blocked by the immunizing peptide and they correspond to earlier findings in the literature with different antibodies (Wang et al, J Neurosci. 2002 Jul 1;22(13):5505-15). This protein has a calculated MW of 50.7kDa according to NP_075380.1. Recommended concentration: 0.3-1 μg/ml.

Key Properties

HostGoat
ClonalityPolyclonal
TargetNogo 66 receptor
Molecular Weight50.7
PurificationPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesSupplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
DisclaimerFor research use only

Alternative Names

anti Nogo-66 receptor antibody, anti RTN4R antibody, anti reticulon 4 receptor antibody, anti HGNC:1860 antibody, anti NGR antibody, anti NOGOR antibody, anti UNQ330/PRO526 antibody, anti nogo receptor antibody, anti reticulon 4 receptor precursor antibody, anti OTTHUMP00000197866 antibody

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Nogo 66 receptor Antibody

Western blot analysis of Human Brain lysate using RTN4R antibody

NCBI Gene Details

No NCBI Gene data available

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Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol

Nogo 66 receptor Antibody (orb19023)

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Available Sizes

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100 μg
$ 530.00
DispatchUsually dispatched within 5-10 working days
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