NMDA receptor 1/GRIN1 Antibody

SKU: orb19268

Description

Goat polyclonal antibody to GRIN1

Research Area

Neuroscience

Images & Validation

• Tested Applications: ELISA, IF
• Dilution Range: ELISA: 1:1000, WB: 1-3 μg/ml
• Reactivity: Human, Mouse
• Predicted Reactivity: Canine, Rat
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:1000.WB: Approx 110kDa band observed in Rat Brain lysates (calculated MW of 105kDa according to NP_015566.1). Recommended concentration: 2-4 μg/ml. A minor band of unknown identity was also consistently observed at 23-24kDa. This band was strong in Mouse Brain lysates and faint in Human Brain lysates. The band was successfully blocked by incubation with the immunising peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: NMDA receptor 1 / GRIN1
• Protein Sequence: TQERVNNSNKKE
• Molecular Weight: 99.3; 101; 105
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti GRIN1 antibody, anti NMDA receptor 1 antibody, anti glutamate receptor, ionotropic, N-methyl D-aspartate 1 antibody, anti NMDA1 antibody, anti NMDAR1 antibody, anti NR1 antibody, anti N-methyl-D-aspartate receptor channel, subunit zeta-1 antibody, anti glutamate [NMDA] receptor subunit zeta 1 antibody

Western blot analysis of Rat Brain lysate using GRIN1 antibody

Western blot analysis of Human Amylgada and Rat Brain lysate using GRIN1 antibody

Quick Database Links

Gene Symbol

NMDA receptor 1 / GRIN1

NCBI Gene ID

2902