Neuroligin 2 Antibody

SKU: orb19483

Description

Goat polyclonal antibody to NLGN2

Research Area

Neuroscience

Images & Validation

• Tested Applications: ELISA, FC, IF, WB
• Dilution Range: ELISA: 1:4000, WB: 2-6 μg/ml
• Reactivity: Human
• Predicted Reactivity: Canine, Mouse, Rat
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:4000.WB: Approx 90kDa band observed in Human Pancrease lysates (calculated MW of 90.8kDa according to NP_065846.1). Recommended concentration: 2-6µg/ml. An additional band of unknown identity was also consistently observed at 50kDa. This band was successfully blocked by incubation with the immunising peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported.

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: Neuroligin 2
• Protein Sequence: NPPDTDIRDPGKK
• Molecular Weight: 90.8
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti neuroligin 2 antibody, anti KIAA1366 antibody, anti NLGN2 antibody

orb19483 (2µg/ml) staining of Human Pancrease lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Quick Database Links

Gene Symbol

Neuroligin 2

NCBI Gene ID

57555