Goat anti-Neuroligin 2 Antibody

• Catalog Number: orb19483
• Category: Antibodies
• Description: Goat polyclonal antibody to NLGN2
• Target: Neuroligin 2
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Canine, Human, Mouse, Rat
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Protein Sequence: NPPDTDIRDPGKK
• RRID: AB_10752010
• MW: 90.8
• Tested applications: ELISA, FC, IF, WB
• Dilution range: ELISA: 1:4000, WB: 2-6 μg/ml
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:4000.WB: Approx 90kDa band observed in Human Pancrease lysates (calculated MW of 90.8kDa according to NP_065846.1). Recommended concentration: 2-6µg/ml. An additional band of unknown identity was also consistently observed at 50kDa. This band was successfully blocked by incubation with the immunising peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported.
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: anti neuroligin 2 antibody, anti KIAA1366 antibody
• Note: For research use only
• Entrez: 57555

orb19483 (2µg/ml) staining of Human Pancrease lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.