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Neurogranin precursor Antibody

SKU: orb20360

Description

Goat polyclonal antibody to NRGN

Research Area

Neuroscience

Images & Validation

Tested ApplicationsELISA, IHC, WB
Dilution RangeELISA: 1:8000, WB: 0.1-0.3 μg/ml, IHC-P: 5 μg/ml
ReactivityHuman
Predicted ReactivityBovine, Canine, Mouse
Application Notes
ELISA: Peptide ELISA: antibody detection limit dilution 1:8000.WB: Approx 15kDa band observed in Human Brain (Cerebral Cortex and Hippocampus), and in Mouse Brain and Rat Brain lysates (calculated MW of 7.6kDa according to NP_006167.1). There is also an additional 16kDa band. The observed molecular weights correspond to earlier findings in literature with different antibodies (Li et al, J Biol Chem. 1999 Jan 15;274(3):1294-300; PMID: 9880498). Recommended concentration: 0.1-0.3 μg/ml.

Key Properties

ClonalityPolyclonal
TargetNeurogranin precursor
Protein SequenceKIKSGERGRKG
Molecular Weight7.62
PurificationPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesSupplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

anti NRGN antibody, anti neurogranin (protein kinase C substrate, RC3) antibody, anti RC3 antibody, anti hng antibody, anti calmodulin-binding protein antibody, anti neurogranin antibody, anti protein kinase C substrate antibody

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NCBI Gene Details

No NCBI Gene data available

Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
IHC
Immunohistochemistry
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol

Available Sizes

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100 μg
$ 560.00
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Free Secondary Antibody (20 ul)0/0

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