Goat anti-Neurogranin precursor Antibody

• Catalog Number: orb20360
• Category: Antibodies
• Description: Goat polyclonal antibody to NRGN
• Target: Neurogranin precursor
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Bovine, Canine, Human, Mouse
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Protein Sequence: KIKSGERGRKG
• RRID: AB_10749794
• MW: 7.62
• Tested applications: ELISA, IHC, WB
• Dilution range: ELISA: 1:8000, WB: 0.1-0.3 μg/ml, IHC-P: 5 μg/ml
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:8000.WB: Approx 15kDa band observed in Human Brain (Cerebral Cortex and Hippocampus), and in Mouse Brain and Rat Brain lysates (calculated MW of 7.6kDa according to NP_006167.1). There is also an additional 16kDa band. The observed molecular weights correspond to earlier findings in literature with different antibodies (Li et al, J Biol Chem. 1999 Jan 15;274(3):1294-300; PMID: 9880498). Recommended concentration: 0.1-0.3 μg/ml.
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: anti NRGN antibody, anti neurogranin (protein kina
• Note: For research use only
• Entrez: 4900

Immunohistochemical analysis of formalin-fixed and paraffin-embedded Human Cortex tissue using NRGN antibody

Western blot analysis of Human Brain (Frontal Cortex) lysate using NRGN antibody

Immunohistochemical staining of Human Cortex using NRGN antibody

Western blot analysis of Human Cerebellum (Lane1) and Rat Brain (Lane2) lysates using NRGN antibody

Western blot analysis of Mouse Brain lysate using NRGN antibody