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Neurofascin (aa877-890) Antibody

SKU: orb22506

Description

Goat polyclonal antibody to NFASC

Images & Validation

Tested ApplicationsELISA, WB
Dilution rangeELISA: 1:16000, WB: 0.5-2 μg/ml
ReactivityHuman
Predicted ReactivityBovine, Canine, Mouse, Rat
Application Notes
WB: Approx 170kDa band observed in Human Lung and Rat Brain lysates (calculated MW of 138kDa according to NP_001005388.2). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Kirschbaum et al, J Biol Chem. 2009 Oct 16;284(42):28533-42. PMID: 19666467). Recommended concentration: 0.5-2 μg/ml.

Key Properties

HostGoat
ClonalityPolyclonal
TargetNeurofascin (aa877-890)
Molecular Weight138; 134; 132; 132;
PurificationPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesSupplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
DisclaimerFor research use only

Alternative Names

anti NFASC antibody, anti neurofascin antibody, anti DKFZp686P2250 antibody, anti FLJ46866 antibody, anti KIAA0756 antibody, anti NF antibody, anti NRCAML antibody, anti OTTHUMP00000065316 antibody, anti OTTHUMP00000065319 antibody, anti OTTHUMP00000065323 antibody, anti OTTHUMP00000065324 antibody, anti neurofascin homolog antibody
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Neurofascin (aa877-890) Antibody

orb22506 (0.5ug/ml) staining of Human Lung lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

NCBI Gene Details

No NCBI Gene data available

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Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol

Neurofascin (aa877-890) Antibody (orb22506)

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Available Sizes

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100 μg
$ 500.00
DispatchUsually dispatched within 5-10 working days
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