NET1/ARHGEF8 (Internal) Antibody

SKU: orb19257

Description

Goat polyclonal antibody to NET1

Research Area

Epigenetics, Neuroscience

Images & Validation

• Tested Applications: ELISA, IHC
• Dilution Range: ELISA: 1:16000, WB: 0.3 μg/ml, IHC-P: 2.5µg/ml
• Reactivity: Human
• Predicted Reactivity: Canine, Mouse, Rat
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:16000.IHC: In paraffin embedded Human Breast shows cytoplasm staining of myoepithelial cells of ducts. Recommended concentration, 2.5ug/ml.WB: Preliminary experiments gave an approx 80-90kDa double band in Human Placenta lysates after 0.3 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 67.7kDa according to NP_001040625.1. The 80-90kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: NET1 / ARHGEF8 (Internal)
• Protein Sequence: DKLEYLDEKQRDPR
• Molecular Weight: 67.7; 61.9
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti NET1 antibody, anti ARHGEF8 antibody, anti neuroepithelial cell transforming gene 1 antibody, anti NET1A antibody, anti Rho guanine nucleotide exchange factor (GEF) 8 antibody, anti guanine nucleotide regulatory protein (oncogene) antibody, anti neuroepithelioma transforming gene 1 antibody, anti Net1 proto-oncogene protein antibody, anti small GTP-binding protein regulator antibody

orb19257 (2.5ug/ml) staining of paraffin embedded Human Breast. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Quick Database Links

Gene Symbol

NET1 / ARHGEF8 (Internal)

NCBI Gene ID

10276