NEBL/nebulette Antibody

• Catalog Number: orb20310
• Category: Antibodies
• Description: Goat polyclonal antibody to NEBL
• Target: NEBL / nebulette
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Predicted Reactivity: Canine, Human, Mouse
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Protein Sequence: ELQRLKRTQE
• RRID: AB_10748170
• MW: 116, 31.2
• Tested applications: ELISA
• Dilution range: ELISA: 1:4000, WB: 1 μg/ml
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:4000.WB: Preliminary experiments gave an approx 30kDa band in Human Brain (Frontal Cortex) lysates, but not in Human, Mouse and Rat Heart lysates after 1 μg/ml antibody staining. This protein has a calculated MW of 31.2kDa according to NP_998734.1. The 30kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: anti NEBL antibody, anti nebulette antibody, anti
• Research Area: Cardiovascular Disease, Epigenetics
• Note: For research use only
• Entrez: 10529