Goat anti-NANOS1 Antibody

• Catalog Number: orb19035
• Category: Antibodies
• Description: Goat polyclonal antibody to NANOS1
• Target: NANOS1
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Human
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Protein Sequence: SARDGPPGKKLR
• RRID: AB_10767257
• MW: 30.2
• Tested applications: ELISA, WB
• Dilution range: ELISA: 1:64000, WB: 1-3 μg/ml
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:64000.WB: Approx 35-37kDa band observed in Human Testis lysates (calculated MW of 30.2kDa according to NP_955631.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Clarke et al, Hum Mol Genet. 2004 Apr 1;13(7):727-39. Epub 2004 Feb 12, PMID: 14962983). Recommended concentration: 1-3 μg/ml.
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: anti NANOS1 antibody, anti nanos homolog 1 (Drosop
• Note: For research use only
• Entrez: 340719

orb19035 (0.3ug/ml) staining of Human Testis lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.