Goat anti-NANOG (C Terminus) Antibody

SKU: orb19481

Description

Goat polyclonal antibody to NANOG

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Dilution range: ELISA: 1:128000, WB: 0.05-0.1μg/ml, IHC-P: 1-2μg/ml
• Reactivity: Human
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:128000.IHC: In paraffin embedded Human Testis shows nuclear staining in spermatogonia. Recommended concentration, 1-2ug/ml.WB: Approx 35kDa band observed in nuclear lysates of cell line HeLa (calculated MW of 34.6kDa according to NP_079141.1). Recommended concentration: 0.05-0.1 μg/ml. An additional band of approx. 37kDa is observed and is consistent with the observations of higher molecular weight bands regularly found with antibodies from other sources.

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: NANOG (C Terminus)
• Molecular Weight: 34.6
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Disclaimer: For research use only

Alternative Names

anti NANOG antibody, anti Nanog homeobox antibody, anti homeobox transcription factor Nanog antibody, anti homeobox transcription factor Nanog-delta 48 antibody

Western blot analysis of HeLa (nuclear) cell lysate using NANOG antibody

Western blot analysis of paraffin embedded Human Testis using NANOG antibody

Quick Database Links

Gene Symbol

NANOG (C Terminus)

NCBI Gene ID

79923