Goat anti-NANOG (C Terminus) Antibody

• Catalog Number: orb19481
• Category: Antibodies
• Description: Goat polyclonal antibody to NANOG
• Target: NANOG (C Terminus)
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Human
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Protein Sequence: DLFLNYSMNMQPED
• RRID: AB_10922445
• MW: 34.6
• Tested applications: ELISA, IHC, WB
• Dilution range: ELISA: 1:128000, WB: 0.05-0.1μg/ml, IHC-P: 1-2μg/ml
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:128000.IHC: In paraffin embedded Human Testis shows nuclear staining in spermatogonia. Recommended concentration, 1-2ug/ml.WB: Approx 35kDa band observed in nuclear lysates of cell line HeLa (calculated MW of 34.6kDa according to NP_079141.1). Recommended concentration: 0.05-0.1 μg/ml. An additional band of approx. 37kDa is observed and is consistent with the observations of higher molecular weight bands regularly found with antibodies from other sources.
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: anti NANOG antibody, anti Nanog homeobox antibody,
• Note: For research use only
• Entrez: 79923

Western blot analysis of HeLa (nuclear) cell lysate using NANOG antibody

Western blot analysis of paraffin embedded Human Testis using NANOG antibody