Goat anti-MYF5 Antibody

SKU: orb19809

Description

Goat polyclonal antibody to MYF5

Images & Validation

• Tested Applications: ELISA, WB
• Dilution range: ELISA: 1:32000, WB: 0.5-1.5 μg/ml
• Reactivity: Human
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.WB: Approx 28kDa band observed in Human Skeletal Muscle lysates (calculated MW of 28.4kDa according to NP_005584.1). Recommended concentration: 0.5-1.5 μg/ml. An additional band of 26kDa was consistently observed, however this band was not blocked by the immunizing peptide and it is therefore a non-specific signal. We call for caution when used for other assays than Western blot.

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: MYF5
• Molecular Weight: 28.4
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Disclaimer: For research use only

Alternative Names

anti MYF5 antibody, anti myogenic factor 5 antibody

orb19809 (0.5ug/ml) staining of Muscle lysate (35ug protein in RIPA buffer) with (B) and without (A) blocking with the immunising peptide. Primary incubation was 1 hour. Detected by chemiluminescence.

Quick Database Links

Gene Symbol

MYF5

NCBI Gene ID

4617