MSH5 Antibody

• Catalog Number: orb19935
• Category: Antibodies
• Description: Goat polyclonal antibody to MSH5
• Target: MSH5
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Human
• Predicted Reactivity: Mouse, Rat
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Protein Sequence: GDLHCEIRDQET
• RRID: AB_10928181
• MW: 94.7; 93.0; 92.9
• Tested applications: ELISA, IHC
• Dilution range: ELISA: 1:64000, WB: 0.1 μg/ml, IHC-P: 2.5-3.75 μg/ml
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:64000.WB: Preliminary experiments gave an approx 38kDa band in Human Heart, Tonsil and Thymus lysates after 0.1 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 92.9kDa according to the smallest reported isoform NP_002432.1. The 38kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: anti MSH5 antibody, anti mutS homolog 5 (E. coli)
• Research Area: Cancer, DNA Damage
• Note: For research use only
• Entrez: 4439

Immunohistochemical staining of Human Testis using MSH5 antibody