Monoglyceride Lipase Antibody

SKU: orb19939

Description

Goat polyclonal antibody to MGLL

Images & Validation

• Tested Applications: ELISA, FC, IF, IHC, WB
• Dilution range: ELISA: 1:128000, WB: 0.3-1 μg/ml, IHC-P: 5 μg/ml
• Reactivity: Human, Mouse
• Predicted Reactivity: Bovine, Canine, Rat
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.WB: Approx. 30kDa band observed in Human Adipose and Brain (Frontal Cortex) lysates (calculated MW of 33.3kDa according to NP_001003794.1). In transfected HEK293 transiently expressing MGLL a band of approx. 36kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0.5-1.5 μg/ml.

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: Monoglyceride Lipase
• Molecular Weight: 34.3; 33.3; 31.1
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Concentration: 0.5-1.5 μg/ml
• Disclaimer: For research use only

Alternative Names

anti monoglyceride lipase antibody, anti HU-K5 antibody, anti MGLL antibody, anti MGL antibody, anti lysophospholipase-like antibody

Western blot analysis of Human Frontal Cortex (Lane 1) and Mouse Adipose (Lane 2) lysates using MGLL antibody

Immunohistochemical staining of Human Liver using MGLL antibody

Immunohistochemical staining of Human Liver using MGLL antibody

Quick Database Links

Gene Symbol

Monoglyceride Lipase

NCBI Gene ID

11343