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Catalog Number | orb19095 |
---|---|
Category | Antibodies |
Description | Goat polyclonal antibody to MAOA |
Species/Host | Goat |
Clonality | Polyclonal |
Tested applications | ELISA, IHC, WB |
Reactivity | Human |
Dilution range | ELISA: 1:64000, WB: 0.1-0.3 μg/ml, IHC-P: 5 μg/ml |
Conjugation | Unconjugated |
MW | 60 |
Target | Monoamine Oxidase A |
Entrez | 4128 |
Protein Sequence | DAPWEAQHADKWDK |
RRID | AB_10747661 |
Storage | Aliquot and store at -20°C. Minimize freezing and thawing. |
Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
Alternative names | anti monoamine oxidase A antibody, anti HGNC:6833 Read more... |
Note | For research use only |
Application notes | ELISA: Peptide ELISA: antibody detection limit dilution 1:64000.WB: Approx 70kDa band observed in Human Heart lysates o(calculated MW of 60.0kDa according to NP_000231.1). In transfected HEK293 transiently expressing MAOA a band of approx. 60 kDa is observed. This band is not observed in the non-transfected HEK293. The user is warned for non-specific background in Western blot. Recommended concentration: 0.1-0.3 μg/ml. |
Expiration Date | 12 months from date of receipt. |
orb19095 (0.3ug/ml) staining of human heart lysate (35ug protein in RIPA buffer) with (B) and without (A) blocking with the immunising peptide. Primary incubation was 1 hour. Detected by chemiluminescence.
HEK293 overexpressing MAOA (RC207276) and probed with orb19095 (mock transfection in first lane), tested by Origene.
Immunohistochemical staining of Human Placenta using MAOA antibody
Immunohistochemical staining of Human Small Intestine using MAOA antibody