MKRN1 (aa105-118) Antibody

SKU: orb125167

Description

Goat Polyclonal to MKRN1. NP_038474.2; NP_001138597.1, PMID: 15805468 and GeneID: 23608.

Images & Validation

• Tested Applications: ELISA, FC, IF, WB
• Dilution Range: ELISA: 1:64000, WB: 1-3 μg/ml
• Reactivity: Human, Mouse
• Predicted Reactivity: Bovine, Canine, Porcine
• Application Notes:
  WB: Approx 50kDa band observed in fetal Mouse Brain lysates and 35kDa plus fainter 50kDa bands in adult Mouse Brain lysates (calculated MW of 52.9kDa according to NP_061280.2). The observed 35kDa band corresponds to the calculated MW of human isoform 2 (35.2k. Notes: Purchase the +ve control! The transfected lysate used to QC this product is available for sale.

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: MKRN1 (aa105-118)
• Protein Sequence: RYEHSKPLKQEEAT
• Molecular Weight: 53.3; 35.2
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti MKRN1 antibody, anti makorin ring finger protein 1 antibody, anti FLJ21334 antibody, anti RNF61 antibody, anti E3 ubiquitin-protein ligase makorin-1 antibody, anti OTTHUMP00000212121 antibody, anti OTTHUMP00000212124 antibody, anti RING finger protein 61 antibody

0.5 μg/ml staining of THP-1 cell lysate (35 μg protein in RIPA buffer). Detected by chemiluminecence.

0.5 μg/ml staining of Human Testes lysate (35 μg protein in RIPA buffer). Detected by chemiluminecence.

Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 μg/ml) followed by Alexa Fluor 488 secondary antibody (2 μg/ml), showing nuclear and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 μg/ml) followed by Alexa Fluor 488 secondary antibody (2 μg/ml).

Flow cytometric analysis of paraformaldehyde fixed THP-1 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 μg/ml) followed by Alexa Fluor 488 secondary antibody (1 μg/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Quick Database Links

Gene Symbol

MKRN1 (aa105-118)

NCBI Gene ID

23608