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Item 1 of 4
Item 1 of 4
Goat anti-MKRN1 (aa105-118) Antibody
Catalog Number: orb125167
| Catalog Number | orb125167 |
|---|---|
| Category | Antibodies |
| Description | Goat Polyclonal to MKRN1. NP_038474.2; NP_001138597.1, PMID: 15805468 and GeneID: 23608. |
| Species/Host | Goat |
| Clonality | Polyclonal |
| Tested applications | ELISA, FC, IF, WB |
| Reactivity | Bovine, Canine, Human, Mouse, Porcine |
| Dilution range | ELISA: 1:64000, WB: 1-3 μg/ml |
| Conjugation | Unconjugated |
| MW | 53.3; 35.2 |
| Target | MKRN1 (aa105-118) |
| Entrez | 23608 |
| Protein Sequence | RYEHSKPLKQEEAT |
| Storage | Aliquot and store at -20°C. Minimize freezing and thawing. |
| Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
| Alternative names | anti MKRN1 antibody, anti makorin ring finger prot Read more... |
| Note | For research use only |
| Application notes | WB: Approx 50kDa band observed in fetal Mouse Brain lysates and 35kDa plus fainter 50kDa bands in adult Mouse Brain lysates (calculated MW of 52.9kDa according to NP_061280.2). The observed 35kDa band corresponds to the calculated MW of human isoform 2 (35.2k. Notes: Purchase the +ve control! The transfected lysate used to QC this product is available for sale. Please email [email protected] to request datasheet, pricing and delivery information. |
| Expiration Date | 12 months from date of receipt. |
0.5 μg/ml staining of THP-1 cell lysate (35 μg protein in RIPA buffer). Detected by chemiluminecence.
0.5 μg/ml staining of Human Testes lysate (35 μg protein in RIPA buffer). Detected by chemiluminecence.
Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 μg/ml) followed by Alexa Fluor 488 secondary antibody (2 μg/ml), showing nuclear and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 μg/ml) followed by Alexa Fluor 488 secondary antibody (2 μg/ml).
Flow cytometric analysis of paraformaldehyde fixed THP-1 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 μg/ml) followed by Alexa Fluor 488 secondary antibody (1 μg/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
