mKIAA0319 (mouse) Antibody

• Catalog Number: orb19876
• Category: Antibodies
• Description: Goat polyclonal antibody to D130043K22Rik
• Target: mKIAA0319 (mouse)
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Mouse
• Predicted Reactivity: Rat
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Protein Sequence: QGKIKQENKPTLH
• RRID: AB_10753166
• MW: 118
• Tested applications: ELISA, WB
• Dilution range: ELISA: 1:128000, WB: 0.5-1.5 μg/ml
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:128000.WB: Approx 150kDa band observed in Mouse Brain lysates (calculated MW of 118kDa according to Mouse NP_001074520.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Velayos-Baeza, Hum Mol Genet. 2008 Mar 15;17(6):859-71; PMID: 18063668). Recommended concentration: 0.5-1.5 μg/ml. An additional band of unknown identity was also consistently observed at 25kDa. This band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: anti mKIAA0319 antibody, anti similar to KIAA0319
• Note: For research use only

orb19876 (0.5ug/ml) staining of Mouse Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.