MICS1/GHITM Antibody

• Catalog Number: orb20456
• Category: Antibodies
• Description: Goat polyclonal antibody to GHITM
• Target: MICS1 / GHITM
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Human
• Predicted Reactivity: Bovine, Canine, Mouse, Porcine, Rat
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Protein Sequence: PSREYATKTRIGIR
• RRID: AB_10749798
• MW: 37.2
• Tested applications: ELISA, WB
• Dilution range: ELISA: 1:8000, WB: 0.01-0.1 μg/ml
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:8000.WB: Approx 35kDa band observed in HeLa cell lysates (calculated MW of 37.2kDa according to Human NP_055209.2). Recommended concentration: 0.1-0.5 μg/ml.
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: anti-DERP2 antibody, anti-Ghitm antibody, anti-GHI
• Research Area: Apoptotic, Cancer, Neuroscience, Signaling Pathway
• Note: For research use only
• Entrez: 27069

orb20456 (0.3ug/ml) staining of HeLa cell lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Western blot analysis of staining of Human Cerebellum (A), Duodenum (B) and Heart (C) cell lysates using GHITM antibody.