MIC1/C18orf8 Antibody

SKU: orb19578

Description

Goat polyclonal antibody to C18orf8

Research Area

Epigenetics

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Dilution Range: ELISA: 1:32000, WB: 1-3 μg/ml, IHC-P: 5-2=10µg/ml
• Reactivity: Human
• Predicted Reactivity: Canine, Mouse, Rat
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.WB: Preliminary experiments gave an approx 90kDa band in Human Bone Marrow, Spleen and Tonsil lysates after 0.1 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 75kDa according to NP_037458.3. The 90kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported An additional band of 55kDa was consistently observed, however this band was not blocked by the immunizing peptide and it is therefore a non-specific signal. We call for caution when used for other assays than Western blot.

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: MIC1 / C18orf8
• Protein Sequence: DKLNHEYKKYLDAEQ
• Molecular Weight: 75
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti C18orf8 antibody, anti chromosome 18 open reading frame 8 antibody, anti FLJ23453 antibody, anti HsT2591 antibody, anti MIC1 antibody, anti colon cancer associated protein Mic1 antibody, anti colon cancer-associated protein Mic1 antibody

Western blot analysis of Human Breast lysate using C18orf8 antibody

Immunohistochemical staining of Human Small Intestine using C18orf8 antibody

Quick Database Links

Gene Symbol

MIC1 / C18orf8

NCBI Gene ID

29919