Goat anti-MDM2 (isoform) Antibody

• Catalog Number: orb19219
• Category: Antibodies
• Description: Goat polyclonal antibody to MDM2
• Target: MDM2 (isoform)
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Bovine, Canine, Feline, Human, Porcine, Rat
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Protein Sequence: DELSGERQRKRHKSD
• RRID: AB_10750667
• MW: 56
• Tested applications: ELISA, WB
• Dilution range: ELISA: 1:64000, WB: 0.3-1.0 μg/ml
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:64000.WB: Approx 55kDa band observed in Human Liver lysates (calculated MW of 56.0kDa according to NP_002383.2). In transfected HEK293 transiently expressing MDM2 bands of approx. 48kDa, 60KDa, 70kDa and 90kDa are observed. These bands are not observed in the non-transfected HEK293. The 80kDa band is endogenously epressed in the negative control. Recommended concentration: 0.3-1.0 μg/ml.
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: MDM2, Mdm2, transformed 3T3 cell double minute 2,
• Note: For research use only
• Entrez: 4193

orb19219 (0.3ug/ml) staining of Human Liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

HEK293 overexpressing MDM2 (RC219518) and probed with orb19219 (mock transfection in first lane), tested by Origene.