MDM2 (isoform) Antibody

SKU: orb19219

Description

Goat polyclonal antibody to MDM2

Images & Validation

• Tested Applications: ELISA, WB
• Dilution range: ELISA: 1:64000, WB: 0.3-1.0 μg/ml
• Reactivity: Human
• Predicted Reactivity: Bovine, Canine, Feline, Porcine, Rat
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:64000.WB: Approx 55kDa band observed in Human Liver lysates (calculated MW of 56.0kDa according to NP_002383.2). In transfected HEK293 transiently expressing MDM2 bands of approx. 48kDa, 60KDa, 70kDa and 90kDa are observed. These bands are not observed in the non-transfected HEK293. The 80kDa band is endogenously epressed in the negative control. Recommended concentration: 0.3-1.0 μg/ml.

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: MDM2 (isoform)
• Molecular Weight: 56
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Disclaimer: For research use only

Alternative Names

MDM2, Mdm2, transformed 3T3 cell double minute 2, p53 binding protein (mouse), HGNC:6973, MGC71221, hdm2, mouse double minute 2 homolog, mouse double minute 2, human homolog of, p53-binding protein, p53-binding protein MDM2, ubiquitin-protein ligase E3 Md

orb19219 (0.3ug/ml) staining of Human Liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

HEK293 overexpressing MDM2 (RC219518) and probed with orb19219 (mock transfection in first lane), tested by Origene.

Quick Database Links

Gene Symbol

MDM2 (isoform)

NCBI Gene ID

4193